Primer

Part:BBa_K4047013:Design

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-09-08)


dSBPase_glpX_DS-fwd


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Overlap sequence sourced from pXWK3-glgC Supplemental Table S2 from https://academic.oup.com/plphys/article/182/1/507/6116245

Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class II at the locus tag Synpcc7942_0505

https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=491830&to=492867&report=fasta&strand=2

References